Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy.

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Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy.

Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staining. There was improved contrast between bacilli and the background in smears ...

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Examination of smears for tubercle bacilli by fluorescence microscopy.

I n underdeveloped countries, laboratory faci l i ties for the bacterio­ logical d iagnosis of tubercu losis are, at present , very l imited . Cultural methods are un l i kely to be used on a large scale for many years to come. It is , therefore, important to investigate the most economical method of examin ing smears for tubercle baci l l i . Fluorescence microscopy was introduced by H agemann...

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comparison of the value of two different sputum staining for diagnosis of acid fast bacilli

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...

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Relationship between CT Findings of Pulmonary Tuberculosis and Acid-Fast Bacilli Sputum Smears

Background and purpose: Computed tomography (CT) scan is of great help in confirming the diagnosis of tuberculosis (TB) before the sputum test result. The purpose of this study was to investigate the relationship between pulmonary involvement patterns in CT scans of TB patients with positive sputum smears. Materials and methods: This retrospective descriptive-analytical study was conducted usi...

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Safer staining method for acid fast bacilli.

To develop a method for staining acid fast bacilli which excluded highly toxic phenol from the staining solution. A lipophilic agent, a liquid organic detergent, LOC High Studs, distributed by Amway, was substituted. The acid fast bacilli stained red; nuclei, cytoplasm, and cytoplasmic elements stained blue on a clear background. These results compare very favourably with acid fast bacilli stai...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1976

ISSN: 0021-9746

DOI: 10.1136/jcp.29.10.931